metamorph software (MetaMorph Inc)
90
Structured Review
MetaMorph Inc
metamorph software
Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+software/metamorph+software/pmc12182373-62-0-20
Average 90 stars, based on 1 article reviews
Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+software/metamorph+software/pmc12182373-62-0-20
Average 90 stars, based on 1 article reviews
metamorph software - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation"
Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation
Journal: Molecular Metabolism
doi: 10.1016/j.molmet.2025.102175
Figure Legend Snippet: Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.
Techniques Used: Western Blot, Immunofluorescence, Staining
Figure Legend Snippet: Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.
Techniques Used: RNA Sequencing, Immunofluorescence, Staining, Control, Mutagenesis, Expressing
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Article Title: Inferring Spatially Varying Bending Stiffness of Biopolymers with Deep-Learning Approach Article Snippet: Article Title: Article Snippet: This Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click Article Title: Supporting Information for Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri Article Snippet: Fluorescence microscopy was performed on a ZEISS IMAGER Z2 upright microscope, equipped with a CoolSNAP HQ2 camera (PHOTOMETRICS, roper Scientific), DAPI, GFP, Article Title: RIPOR2 promotes multinucleation of melanoma cells downstream of the RAS/ERK oncogenic pathway Article Snippet: Images of immunofluorescence staining were captured with a fluorescent microscope Leica Inverted 6000 using MetaMorph software. Labeling:Article Title: Supporting Information for DNA Damage-induced Proteasome Phosphorylation Controls Substrate Recognition and Facilitates DNA Repair Article Snippet: After 24 h, laser micro-irradiation was performed using the computercontrolled MicroPoint laser ablation system (Photonics Instruments; 365 nm, 20 Hz) connected to a Nikon Eclipse Ti-E inverted microscope (60X oil-immersion objective) (9). Article Title: Borg3 controls septin polymerization for primary cilia formation Article Snippet: All Article Title: The transcription factor ZFP64 promotes activity-dependent synapse elimination during postnatal cerebellar development Article Snippet: Sections were washed and incubated for 2 h at room temperature with Alexa 488-, Cy3-, and Alexa 647-labeled species-specific secondary antibodies (Jackson ImmunoResearch; Thermo Fisher Scientific). Article Title: Inferring Spatially Varying Bending Stiffness of Biopolymers with Deep-Learning Approach Article Snippet: Article Title: Article Snippet: This Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click Article Title: Supporting Information for Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri Article Snippet: Fluorescence microscopy was performed on a ZEISS IMAGER Z2 upright microscope, equipped with a CoolSNAP HQ2 camera (PHOTOMETRICS, roper Scientific), DAPI, GFP, Article Title: RIPOR2 promotes multinucleation of melanoma cells downstream of the RAS/ERK oncogenic pathway Article Snippet: Images of immunofluorescence staining were captured with a fluorescent microscope Leica Inverted 6000 using MetaMorph software. |